| 质粒名称: | pCox8a-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000758 |
| 产品用途: | Expresses Cox8a-mScarlet3-H in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Cox8a, mScarlet3-H |
| 物种来源: |
H.sapiens, Synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pCox8a-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCox8a-mScarlet3-H (aka mYongHong)载体质粒基本信息
pCox8a-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pCox8a-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
载体图谱.png)
pCox8a-mScarlet3-H (aka mYongHong)质粒载体简介
pCox8a-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pCox8a-mScarlet3-H (aka mYongHong) 4822 bp DNA circular SYN 30-DEC-2024
DEFINITION TSN20241013-0571-00010_758_20241014-BAN4_A04_A04_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4822)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4822
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 173..247
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
CDS 269..340
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="SVLTPLLLRGLTGSARRLPVPRAK"
regulatory 368..377
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 374..1063
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
polyA_signal 1186..1307
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1314..1769)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1796..1900
/gene="bla"
/label=AmpR promoter
promoter 1902..2259
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2110..2245
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2294..3088
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3320..3367
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3696..4284
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 4459..4762
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter join(4763..4822,1..144)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
ORIGIN
1 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
61 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
121 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcatgtccgt
181 cctgacgccg ctgctgctgc ggggcttgac aggctcggcc cggcggctcc cagtgccgcg
241 cgccaagatc cattcgttgg gggatctgtc cgtcctgacg ccgctgctgc tgcggggctt
301 gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag atccattcgt tgggggatcc
361 accggtcgcc accatggata gcaccgaggc agtgatcaag gagttcatgc ggttcaaggt
421 gcacatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
481 cccctacgag ggcacccaga ccgccaagct gagggtgacc aagggtggcc ccctgccctt
541 ctcctgggac atcctgtccc ctcagttcat gtacggctcc agggccttca cgaagcaccc
601 cgccgacatc cccgactact ggaagcagtc cttccccgag ggcttcaagt gggagcgcgt
661 gatgaacttc gaggacggcg gcgccgtgtc cgtggcccag gacacctccc tggaggacgg
721 caccctgatc tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat
781 gcagaagaag acaatgggct gggaagcatc caccgagcgg ttgtaccccg aggacgtcgt
841 gctgaagggc gacattaagc atgccctgcg cctgaaggac ggcggccggt acctggcgga
901 cttcaagacc acctacaggg ccaagaagcc cgtgcagatg cccggcgcct tcaacatcga
961 ccgcaagttg gacatcacat cccacaacga ggactacacc gtggtggaac agtacgaacg
1021 ctccgtggcc cgccactcca ccggcggctc cggtggctcc taaagcggcc gcgactctag
1081 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
1141 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
1201 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
1261 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
1321 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
1381 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
1441 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
1501 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
1561 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
1621 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
1681 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
1741 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
1801 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
1861 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
1921 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
1981 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
2041 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
2101 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
2161 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
2221 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
2281 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
2341 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
2401 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
2461 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
2521 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
2581 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
2641 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
2701 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
2761 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
2821 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
2881 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
2941 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
3001 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
3061 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
3121 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
3181 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
3241 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
3301 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
3361 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
3421 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
3481 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
3541 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
3601 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
3661 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
3721 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
3781 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
3841 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
3901 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
3961 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
4021 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
4081 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
4141 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
4201 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
4261 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
4321 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
4381 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
4441 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
4501 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
4561 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
4621 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
4681 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
4741 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
4801 gcggtttgac tcacggggat tt
//
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